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Aquatic Toxicology Outreach

UC Toxics News: Fall 2001
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Former TSR&TP Trainee Teaches Aquatic Toxicology through K-12 Outreach

by Mika Pringle Tolson

 

 

 

 

 

Pincetich shows students how to prepare and receive samples

Chris Pincetich, shown in the lab coat, trains attendees at the SETAC short course in sample preparation and receiving. Courtesy photo.

High school students in California's Sacramento Valley are getting a taste of aquatic toxicology by gathering local water samples and performing bioassays. Undergraduate interns from UC Davis visit high school science classes and instruct the students on how to gather samples, properly label them, and set up a 48 hour bioassay to determine the water quality of each sample. "Students test for the presence of toxicity in the water samples by monitoring the survival of Ceriodaphnia dubia, a sensitive indicator species that is an important part of the food chain", says Chris Pincetich, director of the Aquatic Toxicology Outreach Program.

Pincetich is a doctoral student in the Pharmacology and Toxicology graduate group at UC Davis, and a former TSR&TP trainee. He has directed the internships since 1999. His research looks at the effects of pesticides on developing fish embryos. It parallells the internship program in that "both programs are trying to determine anthropogenic effects on the aquatic environment", explains Pincetich.

The aquatic toxicology classroom visits began in 1996 as an outreach component to the Center for Environmental Health Sciences (CEHS) at UC Davis. Stephen Clark, another former TSR&TP trainee, created the program and ran it for a year by himself before deciding to turn it into an internship for undergraduates. He found that undergraduates enjoyed the opportunity to teach high school students and that everyone learned from the experience. The program is now co-sponsored by the CEHS and the TSR&TP Ecotoxicology Lead Campus Program. Clark, who received his Ph.D. in 1999, is now the Lab Director of Pacific EcoRisk, an environmental consulting firm in Martinez, California.

Sacramento Area
Schools Involved in the Program:

Adelante High (Roseville)
Woodcreek High
(Roseville)
Roseville High
Laguna Creek High
Center High (Antelope)
Mira Loma High (Sacramento)
Woodland High
Dixon High
Davis High
Emerson Junior High (Davis)

The internship program is offered throughout the academic year, and several undergraduates participate each quarter. Pincetich provides the interns with instruction in toxicology as well as coordinating classroom visits with 10 different area schools (see inset). The interns give a three day curriculum to each science class they visit. The first day consists of an introduction to the basic principles of toxicology through an activity called the Town of Two Lakes. The students are then assigned to gather water samples from local creeks, gutter flows, and ponds. The second day, interns help the students set up their bioassays for each sample. Finally, the interns return to the classes 48 hours later to help the students interpret the results of the assays.

In Davis, high school students have been gathering samples from the same pond for over 5 years, which has provided some interesting toxicity data. Says Pincetich, "I've found good indications of toxicity coming off streets and flowing into West Davis pond." Some dramatic results have been 0% survival of the Ceriodaphnia from samples taken at the drain pipe, and 80% survival from samples 30 feet away. The likely culprit is hydrocarbon contamination from oil and gas spills on the streets.

Pincetich has presented his data from the assays as well as the science class curriculum at scientific meetings of the Society of Toxicology (SOT) and the Society of Environmental Toxicology and Chemistry (SETAC). At the 2001 SOT meeting, 35 teachers from the local community attended Pincetich's presentation of the aquatic toxicology curriculum. This curriculum is available free of charge to teachers who want to try it with their students. Those interested should contact Chris Pincetich at capincetich@ucdavis.edu.



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